Fouad S Salama.
The Effect of Demineralized Bone Powder on the Healing of Tooth Tissues in Dogs
J Pak Dent Assoc Apr - Jun 2001;10(2):81-90.
College of Dentistry, King Saud University, Kingdom of Saudi Arabia

OBJECTIVE: Deminerahzed bone powder (DBP) has been shown to be osteoinductive. The aim of this investigation was to evaluate the effect of DBP on the healing of dental pulp, dentin, cementum and periodontal ligament (PDL) in dogs. MATERIALS AND METHODS: Seven mature dogs were used in this experiment. One dog was sacrificed and used as a bone donor for the preparation of DBP and the other 6 were recipients. A mucoperiosteal flap was reflected and standard perforation was created on the middle third of the roots of anterior teeth of the recipient dogs. The perforations in the right side of the mouth (experimental) were filled with DBP (5mg) while the left side (control) was left unfilled. The animals were perfused and sacrificed at 20 and 45 days following surgery. Specimens of the experimental and control sites were processed for radiographic, stereomicroscopic, histological and scanning electron microscopic (SEM) examinations. Histological evaluation was carried using specific scoring system for inflammatory cell response, vasculature, presence of fibroblasts, new bone apposition, and soft tissue organization. Data were statistically analyzed with non parametric Mann-Whitney test to determine differences in results among the experimental and control groups. RESULTS: The 20 days experimental specimens revealed more cellular fibrous tissues with more blood vessels than control. Also, DBP particles were fused and interconnecting. At 45 days, the connective tissue of the experimental specimens showed increased organization and vascularity as well as new bone formation along the edges of the perforations and in some sections slightly inside the perforations. CONCLUSIONS: DBP is well tolerated by the dental and paradental tissues and did not alter or hinder their repair with no evidence of untoward reaction. DBP creates a more rapid potential for healing but failed to reestablish satisfactory dental and paradental continuity for the period of this study.

Category: Dentistry
Keywords: Demineralized Bone. Root Perforation.

Click here for Full Text Article

This article has been viewed 431 times

Submit your Research Synopsis for Free Expert analysis - Search- Feedback - Drug Index
Post and View Comments on this Article