Saeed Arayne, Najma Sultana, Zakia Bi Bi.
Simultaneous determination of Cefazolin or Ceftizoxime in presence of ascorbic acid from pharmaceutical formulation and human serum by RP-HPLC.
Pak J Pharm Sci Jan ;20(1):56-61.

A rapid, sensitive and specific RP-HPLC method involving HPLC-UV detection was developed and validated for simultaneous determination and quantification of cefazoline or ceftizoxime in presence of ascorbic acid. Chromatography was carried out on a pre-packed Kromasil 100, C18 (5m 25 x 0.46 cm) column using acetonitrile: water (60:40; v/v) as mobile phase at a flow rate of 0.75 ml/minute and effluent were monitored at 265 nm for cefazoline and ascorbic acid while at 270 nm for ceftizoxime. The assay was linear over the concenteration range of 0.05-100g/ml. The method is convenient for determination of cefazoline or ceftizoxime in presence of ascorbic acid with percent recovery ranging from 99.0-100.0% with an inter and intra day CV <3%. The method does not require more than 8 minutes for analysis with good peak resolution and low LOD 0.1 mcg /ml and LOQ 0.3 mcg/ml.

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