Faris Q Alenzi.
BCL-6 prevents mammary epithelial apoptosis and promotes cell survival.
J Pak Med Assoc Jan ;58(9):494-7.

Objective: Proliferation, differentiation and apoptosis are essential processes in the normal functions of the mammary epithelium. The hypothesis examined in this study is that BCL-6 is critically important not only for regulating B cell growth and development, but also for influencing mammary epithelial apoptosis. Methodology: Full length BCL-6 cDNA was retrovirally transduced into EpH-4 cell line. We used flow cytometry of BrdUrd-stained cells to investigate the cell cycle duration of control and transduced cells. TUNEL was used as a marker of apoptosis to find out differences in the frequencies of apoptotic cells in control and transduced cells. Results: Restoration of human BCL-6 into EpH4 cells not only inhibits apoptosis, but also prolongs the cell cycle. The results also indicated that the cell cycle time of BCL-6-transduced EpH4 cells was prolonged by about 3 hours. We found differences in the frequencies of viable and apoptotic cells in cultures of the parent EpH4 cells, control transduced EpH4 cells and BCL-6-transduced EpH4 cells. Conclusion: The results suggest that BCL-6 may be involved in mammary growth or mammary gland cell kinetics, but not in mammary gland development (JPMA 58:494;2008).

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