Saboor M, Ansari Ma, Waheed U, Anwarullah, Umar M.
Prevalence of extended spectrum 946;-LACTAMASE producing enterobacteriaceae in a private hospital of Islamabad, Pakistan.
Int J Rehab Sci Jan ;03(02):18-22.

Background & Objective: Antimicrobial drug resistance has been observed in almost all species of Enterobacteriaceae. Extended spectrum β-lactamase (ESBL) producing organisms secrete β-lactamase enzyme that inhibits the action of certain β-lactam containing drugs likePenicillins,Cephalosporins and Monobactams.Aim of this study was to determine the frequency of ESBL producing Enterobacteriaceae in a private hospital at Islamabad. Material and Methods: The study was conducted at the Kulsum International Hospital, Islamabad from January 2011 to December 2013. Different strains of ESBL colonies appearing on the plates were identified confirmed by API 20E system (BioMérieux).Isolates were tested forpotential ESBL producers using the Cefpodoxime(10Wg) and the Ceftriaxone (30Wg) antibiotic discs.suspected of ESBL production in thepresumptive test were furthered confirmed using thedouble discs synergy according to CLSI guidelines Results: A total of 3,851Enterobacteriaceae isolates were reported from Kulsum International Hospital. Out of which 2,707 (70.29%) were extended spectrum β-lactamaseproducing organism.From ESBL population, 1330 (49.13%) were E. coli, 702 (25.93%) were Pseudomonas, 542 (20.02%) were Klebsiellapneumoniae, 112 (4.14%) were Proteus and 21 (0.78%) were Enterobacter isolates. Conclusion: Findings of this study showed highest frequency of ESBL producing Enterobacteriaceae species. Care, proper dosage of antimicrobial drugs and detection of ESBL producing species are key factors that may help in the prevention of nosocomial infection caused by ESBL producing Enterobacteriaceae species.

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