Ke, Cheng Xian Shi, Jian Zhao Huang, Keli Tang.
Tetramethylpyrazine Effects on the Expression of Scar-Related Genes in Rabbit Benign Biliary Stricture Fibroblasts.
J Coll Physicians Surg Pak Jan ;26(10):813-7.

Objective: To investigate the effects of tetramethylpyrazine (TMP) on transforming growth factor-β1 (TGF- β1), α-smooth muscle actin (α-SMA), and neuronal regeneration related protein (P311) in benign biliary stricture fibroblasts of rabbit. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from April to December 2015. Methodology: Fibroblasts isolated from rabbits following benign biliary stricture were cultured and treated with different concentrations of TMP (0.08, 0.4, and 2.0 mg/ml). TMP-treated cells and non-treated control groups were incubated for 48-hours, and proliferation was assessed using the cell counting kit-8 assay. The mRNA expressions of TGF-β1, α-SMA, and P311 were assessed by quantitative RT-PCR. Protein expressions of TGF-β1 and α-SMA were investigated by Western blotting. Results: Treatment with TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly attenuated both the mRNA and protein expressions of TGF-β1, α-SMA, and P311 (p < 0.05) in a dose-dependent manner. Conclusion: TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly downregulated the mRNA/protein expression of TGF-β1, α-SMA, and P311. Therefore, TMP may be a therapeutic option for the prevention of benign biliary stricture.

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