Salman Ahmed Farsi Kazi, Mohammad Ghiasuddin Shah, Jameel Ahmed Gandahi, Shankar Lal Rathi.
Testicular histomorphology; effects of recombinant human interferon-α-2b in albino rat model.
Professional Med J Jan ;23(10):1209-13.

Objectives: The present study was conducted to investigate the effects of recombinant human interferon-α-2b (rh-INF-α-2b) on testicular histomorphology in adult rat model. Study Design: Experimental study. Place & Duration: Animal house, Sindh Agriculture University Tando Jam and Isra University Hyderabad from January to December 2014. Methodology: 80 adult albino rats were selected according to inclusion and exclusion criteria and divided into 4 groups. Group I: Control rats 0.9% saline, Group II: rhIFN α-2b (3MIU), Group III: rhIFN α-2b (5MIU) and Group IV received rhIFN α-2b (10MIU). The rhIFN α-2b was injected intra-peritoneal (i.p) three times a week for 3 weeks in doses of 3MIU, 5MIU and 10MIU. Animals were euthanized. Orchidectomy was performed and testicles were stored in 10% formaldehyde. 5µ thick tissue sections were stained by Hematoxylin & Eosin (H & E). Results: Atrophic seminiferous tubules with clumping of lining epithelia were noted. Germ cell maturation arrested was prominent; hypervascularity with reduced germ cells and sperm cells were noted in high dose rhIFN α-2b treated groups. Tubular desquamation and thick basement membrane were visible. The sertoli cells and interstitial cells of Leydig counts were increased. Conclusion: It is concluded that the recombinant human interferon-α-2b exerts serious adverse effects on testicular histomorphology.

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