Shazia Akbar Ansari, Rakhshanda Baqai, Muhammad Rizwan Memon, Alaa Abdelhamid, Muhammad Khalil Khan.
Clinical Implication of Methicillin Resistant Staphylococcus Aureus Isolated from Oral Surgical Procedures.
J Pak Dent Assoc Jan ;20(2):109-14.

OBJECTIVE: The aim of this study was to find out the isolation of Methicillin Resistant Staphylococcus aureus (MRSA) in dental patients undergoing various oral surgical procedures. METHODOLOGY: For this study 250 patients between 20-50 years of age were selected, who underwent different oral surgical procedures. 224 (90%) patient had bacteremia (spontaneous or transient) and 26 (10%) patients cultures were negative for bacterial growth. Blood samples were taken immediately after minor oral or dental procedures. To determine the frequency of bacteremia before the surgical procedures (baseline bacteremia), 5ml of blood was obtained from 25% of patients after the administration of local anesthesia, Blood sample were inoculated in the blood culture bottles filled with Tryptic Soya broth (diaphasic media) and incubated at 37°C for 7 days..Then subcultured on blood agar and MacConkey’s agar. The plates were incubated at 37°C aerobically, after 24 hours growth was observed. Colonies were identify by gram’s staining and standard biochemical test. 114 isolated strains of S. aureus were used for detection of Methicillin Resistant S.aureus. Detection of MRSA was done by using ORSA (Oxacillin resistant screening agar base) media , Sensitivity to cefoxitin was also done by disc diffusion method. RESULT: 114 (58%) isolates were S.aureus, Out of 114 S.aureus strains, MRSA were present in 55(48.24%) and cefoxitin resistance was found in 52 % isolates of S.aureus. CONCLUSION: Bacteria capable of causing serious diseases are present in the oral cavity and saliva of different patients without any symptoms. S.aureus and MRSA most frequently spread via dental unit water supply, transiently contaminated hands of health care professionals, improper sterilization technique. Simple infection control precautions such as use of gloves, mask and effective hand hygiene practice, proper strelization of dental equipment, use of sterilized water in dental units and standard infection control precautions should be enforced in dental offices to prevent transmission of MRSA to community and dental practitioner.

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com