Umer Saeed, Imran Anwar Khan, Mulazim Hussain Bukhari, Saman Saeed.
Estimation of Plasma HNE, PGF2α and 8-OHDG Guanosine Levels in patients with Rheumatoid Arthritis in Cosmopolitan Lahore.
Pak J Med Health Sci Jan ;12(4):1457-60.

Aim: Estimation of plasma levels of oxidative stress markers ; HNE, 8-OHdG and Prostaglandin F 2αin patients with rheumatoid arthritis, and highlighting the significance of these biomarkers in the pathogenesis and inflammatory destruction of effected joints in rheumatoid arthritis in the adult population in Lahore Study Design: Case control study Methods: Current study included seventy adult males, and among these individuals, fifty patients were diagnosed as having rheumatoid arthritis with joint complications, while rest 20 individuals served as healthy control subjects. Data collection and analysis: Plasma HNE levels were estimated by utilizing HPLC method, and the eluted fraction contained HNE band with an intense yellow color with absorbance recorded at wavelength 370nm. For the estimation of 8-iso-PGF 2α, EIA standard was added in every test tube, finally wavelength was recorded at 405- 420nm. (8-OHdG) was estimated in the serum of patients with the help of commercially available ELISA kit, resultant solution was incubated and washed, and then conjugate enzyme was added, finally reading the incubation plate at 450nm Results : In the disease group, significantly increased plasma concentrations of HNE and Prostaglandin F 2α (p=0.019) and (p=0.033) respectively were observed ; (9.65+/-1.06 µmol/ml) and (47.56+/-3.15pg/ml) respectively, compared with healthy control subjects (2.06+/-0.05 µmol/ml) and (1.84+/-0.0023 pg/ml) respectively. Interestingly, 8- OHdG concentration in plasma appeared to be most significantly raised (p=0.015 ) in patients with RA (1.52+/-0.016 pg/ml), compared with healthy control subjects (0.023+/-0.001 pg/ml). Conclusion: Current study highlights the significance of inflammatory and oxidative biomarkers in pathogenesis and progression of rheumatoid arthritis in adult population in Lahore. Presented study demonstrated that 8-OHdG levels were most significantly related to joint destruction in patients diagnosed with RA. Plasma levels of HNE and PGF 2α were of less significance in the pathogenesis of RA in the adult selected population

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