Safana Sadaf, Saima Batool, Anila Chughtai, Asma Zafar, Omar Chughtai, Akhtar Sohail Chughtai.
Diagnostic accuracy of immunofluorescence in glomerulonephritis: a single center experience of 150 cases.
Pak J Pathol Jan ;32(2):38-43.

Objective: The objective of this study was to investigate the diagnostic role of immunofluorescence (IF) in accurate diagnosis of various glomerulonephritis (GN) in Pakistani population. Material and Methods: Cross sectional study of one-year duration from January, 2019 to December, 2019 was conducted at histopathology department, Chughtai Institute of Pathology, Lahore, Pakistan. A total of 150 cases of GN were included in this study in which light microscopy (LM) and IF findings were reviewed. All clinical and biochemical data was recorded on a proforma for each case. Statistical methods use to analyze this study included mean +- SD, percentages, ratio, sensitivity, specificity, positive predictive value and negative predictive value. Results: Of the total cases, 79 (52.7%) were males and 71(47.3%) were females. Mean age of the patients was 24.29 +- 15.07 years. IF helped in changing the diagnosis of LM in 28 cases (18.67%). In this study, the most common pattern of GN diagnosed was membranous GN (30.6%) followed by focal segmental glomerulosclerosis (28%), lupus nephritis (11.3%) and membranoproliferative GN (7.3%). The sensitivity of IF was 96.5% and specificity was 94.4%. Conclusion: Our study reinforces the fact that IF in conjunction with LM plays crucial role in the diagnosis of GN along with relevant clinical and biochemical data.

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