Hashaam Akhtar, Gibrail Islam, Syed Umer Jan, Ayesha Nawaz, Samar Akhtar, Rune Hartmann, Hajra Sadia.
Identification of essential regulatory elements responsible for the explicit expression of IL-28Ralpha and their effect on critical SNPs using in-Silico methods..
Pak J Pharm Sci Jan ;28(4-S):1523-32.

IL-28Ralpha and IL10Rbeta collectively construct a fully functional hetero-dimeric receptor for type III interferons (IFNs). IL-28Ralpha is the private chain for type III IFNs since their involvement in any other pathway has not been reported yet and they are highly expressed in response to certain viral attack or cancers. IL-28Ralpha is specific in their expression pattern and it expresses within few cell types only. The regulatory mechanisms governing the expression of IL-28Ralpha at the molecular level are not completely known yet and need to be scrutinized at primary levels. In the present study, various in-silico techniques were applied and it was observed that AP1-2, STAT 1-6, P-53, LyF-1 (lymphoid transcription factor), c-Jun, PU.1, CREB (cAMP response element-binding), PLAG (pleotropic adenoma gene), MYOD (myoblast determination protein 1), NOFL and KLFS as transcription factors that are selected with preference. Interestingly AP-2, c-Jun, LyF-1, STAT, NF-Y and P53 have also been reported in literature recently as some of the key regulatory elements as well. Based on the fact that interlinking between different interferon stimulation genes (ISGs) is also not very clear and induction of one type of interferon can affect the efficacy of the other, we found that IFN-lambda4 induction can increase the expression of IL-28Ralpha, similar to IFN-lambda3 but contrary to type I IFNs, which has either no effect on the expression of IL-28Ralpha or can down regulate its expression at higher concentrations (data not published).

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