Anum Sharif, Mansoor Ishaq Raja, Waqas Hanif, Muhammad Sajid Yazdani, Hamid Iqbal, Noreen Anwar.
Anti-A and Anti-B Antibodies High Titer in Group `O` Blood Donors - a Tertiary Care Hospital Blood Bank Experience.
Pak J Pathol Jan ;33(1):25-8.

Objectives; To determine the frequency of titre anti-A and anti-B antibodies in donors with blood group O. Material and Methods: The study was conducted at Combined Military Hospital Multan from October 2019 to April 2020. This cross-sectional study has conducted after the approval of ethics review committee; A total of 300 individuals with blood group O reported for blood donation during the study period were considered in this study. After their consent, 10 ml whole blood of each donor was collected in two tubes,3 ml collected in Ethylenediaminetetraacetic acid (EDTA)tube and 7 ml in plain tube. Anti-A & anti-B antibodies titers were performed using pooled blood group A and B red cells by tube method at room temperature and 37C. Results: Male consisted of 174 (58%)and female consisted of 126 (42%)with male to female ratio of 1.5:1out of 300 blood donors. The ages of individuals range from 20 to 50 years (32.8 +- 7.2). The frequenciesoftiterofanti-Aantibodiesandanti-Bantibodieswerefoundtobe17.7%and9.0%respectively.Conclusion: Titer of anti-A antibodies is high in considerable number of blood group O donors supports cautious use of Blood group O products in non-O recipients without screening. However, frequency of titer of anti-B antibodies was found to be relatively low.

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