Hajrah Syndeed Pal, Natasha Ali, Muhammad Asif Naveed, Umera Saleem, Yusra Rashid, Mujtaba Ashal Pal, Shabbir Hussain.
Aberrant Expression of CD Markers on Flow Cytometric Analysis in Suspected patients of Leukemias and Lymphomas: a Single-Centered Study.
J Sharif Med Dent Coll Jan ;8(2):88-91.

Objective: To determine the frequencies and pattern of aberrant CD markers expression in Pakistani patients of leukemias and lymphomas. Methodology: It was a cross-sectional study conducted from April to September 2020 at the Department of Hematology and Transfusion Medicine, Aga Khan University Hospital, Karachi. Peripheral blood/bone marrow samples from patients of suspected hematological malignancies subjected to flow cytometric evaluation were included. Samples were lysed, tested for viability, and analyzed through 5-colour flow cytometer (FC500). Data was entered in Statistical Package for the Social Sciences (SPSS) version 22. Results: A total of 262 patients were enrolled. Male to female ratio in the study was determined to be 1.5:1. Further categorization showed acute leukemias comprised 75.78% of the total cases whereas chronic lymphoproliferative disorders were 22.26%. The overall presence of aberrant markers was 22.8% among all cases. The percentage of aberrant markers expression was higher in leukemic patients as compared to lymphoma patients. Conclusion: Aberrant expression of CD markers is a common entity. Their possible presence should be considered and evaluated while determining the lineage for acute leukemias specifically according to the latest WHO criteria through flow cytometry. Keywords: Leukemias. Lymphomas. Flow cytometry.

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