Haniloo A, Massoud J, Rokni M B.
Evaluation and comparison of antigen b-Elisa and antigen b-Immunoblotting in immunodiagnosis of cystic hydatid disease.
Pak J Med Sci Jan ;21(3):352-6.

Objective: Evaluation and comparison of the diagnostic value of ELISA using partial purified antigen B (AgB-ELISA) and antigen B subunits immunoblotting in immunodiagnosis of cystic hydatid disease (CHD). Design: Antigen B was obtained and partially purified from sheep hydatid cyst fluid. Serum samples were collected from surgically confirmed CHD patients, other parasitic disease, patients with malignancies and normal individuals. Sera were analyzed by ELISA using antigen B and immunoblotting based on antigen B subunits. Setting: School of Public Health serum blood bank, Tehran University of Medical Sciences, volunteers and selected CHD patients from different local hospitals. Subject: Serum samples were obtained from 64 surgically confirmed CHD patients, 55 from individuals infected with toxocariasis or fascioliasis, 50 from patients with malignancies and 73 from normal individuals. Main Outcome Measures: The sensitivity, specificity and cross-reaction of comparative tests evaluated. Result: The sensitivity of AgB-ELISA was 89%, while that of 8/12-, 16-kDa immunoblot was 80%. Specificity of AgB-ELISA was 98% and that of 8/12-, 16-kDa immunoblot was 100%. The sensitivity and specificity of ELISA using crude hydatid fluid antigen (CHFAg-ELISA) were 94% and 83%, respectively. Conclusion: AgB-ELISA as well as 8/12-, 16-kDa immunoblot can be convenient in specific and confirmatory diagnosis of CHD.

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com