Khuhawar M Y, Rajper A D, Rind F M A.
Spectrophotometric and liquid chromatographic determination of dopamine from pharmaceutical preparations using acetyl acetone as derivatizing reagent.
Pak J Pharm Sci Jan ;19(4):286-9.

Dopamine (DA) has been determined by spectrophotometry and by liquid chromatography as derivatives of acetylacetone (AA). The liquid chromatography of DA was carried out in the presence of hydroxyproline (HP), octopamine (OP) and tyramine (TY). The separation was obtained from Phenomenex C-18 column 5pm (150 x 4.6 mm id) by isocratic elution with methanol: acetonitrile: water (45: 6: 49v/v/v), UV detection was at 320nm. Linear calibration curves were obtained for each with 2.5-40.0µgml-1 with detection limits in the range 1.8-80ng injection-1 (10µl). The dopamine was determined in pharmaceutical preparations with coefficient of variation (CV) within 0.7-1.5%.

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