Samia Perwaiz, Qamaruddin Barakzi, Badar Jehan Farooqi, Nazia Khursheed, Nasim Sabir.
Antimicrobial susceptibility pattern of clinical isolates of Methicillin Resistant Staphylococcus aureus.
J Pak Med Assoc Jan ;57(1):2-4.

Objective: To study the antimicrobial susceptibility pattern of MRSA isolates from patients in a tertiary care hospital. Methods: This was in-vitro study on MRSA isolates received from clinical samples in the department of microbiology during one year (March 2004- February 2005). All samples were processed by conventional method using sheep blood agar, MacConkey`s agar and Chocolate agar plates. Staphylococci were identified by catalase, coagulase and D`nase tests. Antimicrobial susceptibility testing of all isolates was performed on Mueller-Hinton agar plates by modified Kirby Bauer`s Disc Diffusion method. The disc used Oxacillin (1ug), Cephalexin (10ug), Ofloxacin(5ug), Fusidic acid (10ug), Penicillin ( 10ug), Vancomycin (30ug), Erythromycin (15ug), Gentamicin (10ug), Teicoplanin (30ug), Amikacin (30ug) and Clindamycin (2ug). Results: Our results indicate that out of 190 positive isolates of S. aureus, 82 (43%) were found to be MRSA. These strains were also resistant to many other antistaphylococcal antibiotics. Conclusion: A total of 82 (43%) MRSA were isolated from various clinical samples. Pattern of first line antistaphylococcal antibiotics is changing. Antimicrobial susceptibility testing is crucial in the treatment of these patients (JPMA 57:2;2007).

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