Fatima Noman, Uzma Usman, Altaf Ahmed.
Comparison of disk susceptiblity test of Gram-Negative Rods with MIC for Cefoperazone-Sulbactam Combination..
Infect Dis J Jan ;16(1):3-5.

We performed a comparative study to find out the sensitivity and specificity of disk diffusion (DD) for in vitro susceptibility test of cefoperazone /subactam with MIC against isolates of Acinetobacter. Material and Methods: In-vitro susceptibilities of a total of 42 isolates of Acinetobacter species were performed by MIC (Estrip method) and DD methods. Sensitivity and specificity of the DD was calculated with MIC as standard. Results were analyzed on SPSS version 13.0 and expressed as scattergram. Results: A total of 42 Acinetobacter species were isolated. All were resistant to Cefoperazone alone when checked by DD method. Twenty two were sensitive to Cefoperazone/sulbactam by MIC (< 32/16 µg/ml) and 20 were resistant to Cefoperazone/sulbactam (> 32/16 µg/ml). 19 (86%) of sensitive isolates were also sensitive by DD, 18 (86%) of resistant isolates were also resistant by DD. Sensitivity and Specificity of DD were 86% each. Correlation was significant at the 0.01 level (2-tailed). Among 6/42 discrepancies, 5 were minor and 1 was major. Conclusion: DD method for reporting antimicrobial sensitivity of Acinetobacter species against Cefoperazone/Sulbactam is consistent with MIC with a sensitivity and specificity of 86% each.

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