Ahmad Mohammed Salih.
Cytotoxic effects of carcino-embryonic antigen sensitized human cord blood T cells expressing HSP60 under thermal stress on hela cancer cells.
Rawal Med J Jan ;36(1):53-6.

Objective: To evaluate the in vitro cytotoxic effects of human cord blood T lymphocytes sensitized with carcino embryonic antigen (CEA) expressing HSP60 under thermal stress on human cancer cells of the Hela type. Methods: Human umbilical cord blood mononuclear cells (T lymphocytes and monocytes) were collected, the ratio of monocyte to T lymphocyte was made to 1:10. They were incubated in five separated tissue culture flasks (5 ml cell suspension in each flask) with CEA in a dose 0.5mg/ml and 30 U/ml for 12 days at 37oC and 5% CO2, during which, four of the three culture flasks were exposed to different thermal stress degrees, 37oC, 40oC, 41oC, and 42oC for one hour a day, the other one was not subjected to heat as a negative control. The HSP60 level was measured by ELISA, and T cell cytotoxicity against Hela cancer cells was estimated by lactate dehydrogenase release. Results: HSP60 was found at higher levels in cell suspension exposed to 40 oC and 41 oC (15.4+/-0.4 and 16.1+/-0.2 ng/ml respectively) and there was a clear decline in HSP60 level for the cell suspension exposed to 42oC (2.8+/-0.3 ng/ml). The T cell proliferation increased dramatically and the highest proliferation rate was elicited at day six of incubation with exposure 40 oC and 41 oC when the monocyte-T cell ratio declined to 1:35+/-2 and 1:30+/-2 respectively. T cell mediated cytotoxicity to Hela target cell was found at its maximum. The maximum cytotoxicity percentage mediated by T cells was observed with T cells exposed to 40oC, there was a slight reduction in the T cells cytotoxicity percentage for those exposed to 41oC but there was no significant difference and they possessed an extremely higher anti tumor activity than those subjected to 37, & 42oC. Conclusion: This study shows that in vetro addition of the heat during sensitization of cord blood T cell to cancer associated antigen (CEA) in the range of 40oC to 41oC C has induced both the antigen induced sensitization of T cell and their cytotoxicity against target cancer cells of the Hela type.

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