Naveed Faraz, Ziaul Islam, Rehana Rehman, Sehrish.
Antibiofilm forming activity of naturally occurring compound.
Biomedica Jan ;28(2):171-5.

Introduction: It has been observed that people in general do not pay much attention to maintain proper oral hygiene as a result organisms which are a part of normal flora of oral cavity form oral biofilm over the teeth. When patient is catheterised mostly the catheter remain there for a long period especially in older immobilised very sick patients and over these catheter due to fecal contamination ecoli biofilm formed. Biofilm forming property confers virulence characteristics thus making such bacteria highly resistant to commonly used antibiotics. We analysed the antibiofilm activity of natural occurring substances like dandasa (cassia fistula) and green tea (Camellia sinensis). Material and Methods: Forty streptococcus mutans and 45 ecoli samples were taken from oral biofilm and contaminated urinary catheter respectively and characterised using conventional biochemical, cultural and molecular methods. The biofilm forming activity of these isolates using 96 well plates were checked. Then the dandasa and green tea with those biofilm formers were mixed and observed the antibiofilm forming activity. Results: Both dandasa and green tea in a concentration of 6.2 mg/ml and 12.5 mg/ml respectively show good antibiofilm forming activity against streptococcus mutan and in a concentration of 12.5 mg/ml and 3.1 mg/ml respectively against E. coli. Combinations of dandasa with green tea were found to be not more effective in inhibiting biofilm formation suggesting in different activity with anti-adhesive index of 1.5 and 0.75 against Streptococcus mutans, and ecoli respectively. Conclusion: Streptococcus mutans in oral biofilm and ecoli in urinary catheter biofilm exhibited biofilm formation which is the cause for antibiotic resistance and providing shelter to other organism. Green tea and dandasa provide a good antibiofilm activity individually.

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