Raziuddin Ahmed, Muhammad Asif Durrani, Zahid Sohail, Abid Hussain Chang, Fakharuddin, Muslahuddin Kalar, Sheikh Sajjad Ahmed.
Frequency of Fungal Involvement in Nasal Polyposis.
Pak J Med Health Sci Jan ;6(2):454-8.

Objective: To observe the involvement of fungi in nasal polyp Setting: Study was carried out from September 2008 to May 2010, on twenty six patients in the Department of Microbiology, Basic Medical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), on the specimens (nasal polyps) from tertiary care hospital. Study design: Cross sectional study Method: Specimen were taken from the department of ENT of different tertiary care hospitals and processed for the diagnosis by potassium hydroxide mount and mycological culture in the department of Microbiology, BMSI. Potassium hydroxide mounts was microscopically evaluated for the presence of thread-like branching structures (hyphae) or beaded spherical structures (spores). If present, it was considered as a positive test. Mycological culture was done using Sabouraud's dextrose agar at 25ºC and at 37ºC. Observation for growth was done periodically for 4 weeks; if growth was present then the pathogen was identified by cultural characteristics and microscopy. Statistical method: Descriptive statistics for KOH mount and mycological culture of the type of fungus and organisms were evaluated. Data was collected and results tabulated Result: In 17 specimens the fungal culture was positive and in 2 specimens fungal element was observed only on microscopy while in seven specimens no fungal involvement was seen. Conclusion: There is a high frequency of involvement of fungus in nasal polyps.

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