Misbahul Majeed, Muhammad Tayyib, Zahid Ibrahim, Muhammad Farooq, Allah Ditta, Saeed Ahmad.
Detection of Islet Cell Cytoplasmic Antibodies in First Degree Relatives of Type-1 Diabetic Patients.
Pak J Med Health Sci Jan ;6(3):586-7.

Objectives: To estimate the islet cell cytoplasmic antibodies (ICA) and insulin level in the sera of asymptomatic first degree relatives of patients with type 1 diabetes and compare with normal subjects. Design of study: Cross sectional comparative study Data source: First degree relatives of patient with diagnosed type 1 diabetes attending various hospitals in Lahore. Setting: Department of pathology, postgraduate Medical Institute, Lahore. Methodology: 120 subjects, 60 asymptomatic first degree relatives of type 1 diabetics (Group A) and 60 normal subjects were taken. The blood samples were collected in fasting state. 5 ml blood was collected and serum obtained was used for glucose, insulin and ICA antibodies estimation. Flu: results obtained from both groups were compared Glucose was measured by Oxidase method, insulin level by ELISA method, and ICA by indirect immune fluorecence method by commercially available kits. The students t test was applied for glucose and insulin level while chi square test was applied for ICA to see the level of significance. Conclusions: Islet cell cytoplasmic antibodies (ICA) are positive in 11 subjects (18.3%) of group A and 02 subjects (3.3:%) of group B.

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