Tatheer Zahra, Shadab Ahmed Butt, Muhammad Hussain Ali, Azhar Mubarak, Shahid Jamal.
Role of beta carotene on histomorphology of rat kidneys in subacute apap induced renal damage.
Pak Armed Forces Med J Jan ;64(3):473-8.

Objective: This study was conducted to evaluate the role of beta carotene on histomorphology of rat kidneys in subacute Acetaminophen (APAP)-induced renal damage. Study Design: Lab based randomized control trial Place and Duration of Study: The study was carried out in the department of Anatomy Army Medical College, Rawalpindi; in collaboration with National Institute of Health (NIH), Islamabad for one week in June 2009. Material and Methods: Sixty young adult (4-6 weeks old) Sprague-Dawley rats of both sexes weighing 180-240 g were randomized into three groups. Experimental group A was treated with 700 mg/kg body weight subacute APAP orally once daily for 7 consecutive days. Experimental group B was administered beta carotene 30 mg/kg body weight once daily one hour before 700 mg/kg body weight subacute APAP once daily for 7 consecutive days. Control group C animals were fed NIH laboratory diet. Kidney specimens were collected 24 hours after the last dose. Five micron thick sections of kidney were stained with H&E for histomorphological study. Frequencies and percentages were calculated to describe the variables p values less than 0.05 was considered statistically significant Results: Microscopic examination in experimental group A demonstrated tubular necrosis of level 2 (35% animals) and level 3 (65% animals). Mild vacuolar degeneration was also observed in 90% of the experimental group A animals. In experimental group B, there was statistically significant difference (p-value < 0.001 in levels of renal tubular necrosis (15% animals) and grades of vacuolar degeneration (5% animals) as compared to experimental group A. Findings in experimental group B were not significantly different from that of control group C. Conclusion: Beta carotene has protective role on histomorphology of kidneys in subacute APAP-induced renal damage in rats.

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