Maqsood Ahmad, Shamila Afshan, Madiha Iqbal, Abdul Wadood Khalid, Mehfooz-ur Rahman.
Comparison of calculated method with measured method for low density lipoprotein cholesterol.
J Cardiovascular Dis Jan ;11(3):61-4.

OBJECTIVE: Most of the labs use calculated method for measurement of Low density lipoprotein cholesterol. We conducted this study to know the accuracy of calculated method versus measured method for determination of LDL cholesterol level. MATERIAL AND METHODS: This Cross sectional hospital based study included 153 consecutive healthy individuals aged 17 to 75 years. The patients were selected from Pathology department of Punjab Institute of cardiology, Lahore over a period of 6 months from 1st June 2014 to 31st December 2014. Patients with alcohol consumption, history of smoking, Diabetes Mellitus, liver disease, renal disease or on any lipid lowering agent and without proper fasting were excluded from the study. Data was analyzed using SPSS version 20.0 RESULTS: Out of 153 patients, 90 (58.8 %) were male while 63 (41.3 %) were female patients. The mean age of the participants was 46.46±12.58 years, Low density lipoprotein cholesterol measured by measured method and calculated by Friedewald formula showed uniformity in Triglyceride group ≤100 but there was significant difference in other groups ranging from 101 – 401 mg/dl of TG. It indicated that the Friedewald formula was not rightly predicting the accurate mean Low density lipoprotein cholesterol value as the TG level goes beyond the 100mg/dl. CONCLUSION: Calculated Low density lipoprotein cholesterol is not reliable when serum triglyceride (TG) levels exceed 100mg/dl and measured method is more accurate than calculated method at different TG levels.

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