Abdul Rashid Surahio, Altaf Ahmed Talpur, Abdul Salam Memon, Afzal Junejo, Abdul Aziz Laghari.
Surgical site infections; culture & sensitivity pattern of micro-organisms isolated in a tertiary care hospital.
Professional Med J Jan ;24(1):57-63.

Background: Wound infection has been a major problem in the surgical field since long time. Significant improvements in sterilization, preoperative preparation of patient for surgery, surgical techniques & prophylactic usage of preoperative antibiotics have not been able to eradicate wound infections. Development of wound infection increases the hospital stay, cost of treatment & increase morbidity & mortality associated with surgery. Objectives: To assess type of organism responsible for postoperative wound infection & its drug sensitivity patterns at Public & private sector hospitals of Hyderabad. Study Design: Prospective, descriptive study. Setting: Public & Private Sector Hospitals of Hyderabad, Pakistan. Period: June 2013 to May 2014. Materials & Method: All patients of either sex above the age of 13 years who underwent surgery & developed wound infection were included in the study. Samples to assess culture & sensitivity pattern of organism were taken from infected wounds. Subject’s data was collected on preformed proforma for age, sex, diagnosis, co morbid illness, type of surgery, presence or absence of wound infection, grade of infection, and culture & sensitivity pattern of organism isolated. Results: During this 1 year period total of 424 patients of different pathologies related to General surgery were finally included in analysis. Mean age was 27.35 years with 61.08% were male and 38.91% female. Inguinoscrotal operations were the commonest procedures performed in 113(26.65%) patients followed by Appendicectomy in 102(24.06%) patients. Surgical site infection was noticed in 54(12.74%) patients with 47(13.27%) had this of grade II & above. It includes 23(22.55%) patients of Appendicectomy followed by 09(33.33%) patients of Laparotomy. 47(13.27%) samples were sent for Culture & sensitivity with 41(11.58%) of them showed positive yield. E. coli noticed as commonest organism isolated in 26(63.41%) patients followed by Staphylococcus Aureus in 08(19.51%). Most sensitive antibiotics against noted were Meropenem & Pipracillin with Tazobactum which showed sensitivity to E.coli in 25(96.15%) patients & 24(92.31%) patients respectively while their sensitivity against S. Aureus was 07(87.5%) & 06(75%) patients respectively. Vancomycin was found sensitive against Staphylococcus Aureus in 07(87.5%) patients. Sensitivity of Ampicillin to most of these organisms was found significantly low. Conclusion: Wound infection is responsible for significant morbidity in developing world with the frequency of 15.53% in this study. It puts up significant economic burden on the hospitals.

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