Abid Ali, Kausar Tasneem, Naikzada, Qaisar Inayat.
Comparative Study of Hepatic Serum Enzymes in Albino Rats after treatment with Tamoxifen and Taurine.
Med Forum Jan ;2(9):2-6.

Objective: to compare the mean values of serum hepatic enzymes of albino rats after treatment with tamoxifen and taurine. Study Design: A prospective experimental study. Place and duration of study: This study was conducted in the Department of Anatomy, Khyber Medical College, Peshawar from July 2011 to December 2011. Materials and Methods: Sixty adult female albino rats were divided into four groups (A-control, B-tamoxifen-treated, C tamoxifen plus taurine-treated, and D-taurine treated). Each group was further divided into three subgroups (1, 2, 3) according to the period of treatment which was one, three and six weeks, respectively. Body weights were recorded at the commencement and end of the study period. At the end of the respective period of treatment the animals were sacrificed under deep ether anesthesia. By a midline incision, the anterior abdominal wall was opened. Blood samples were collected with 5cc syringes by cardiac puncture for estimation of serum hepatic enzymes (serum glutamic pyruvate transaminase-SGPT, Serum glutamic oxaloacetic transaminase (SGOT), and serum alkaline phosphatase (ALP). The livers were removed, washed with normal saline, and their weights were recorded. Formalin fixed, paraffin embedded 4 micron thick sections were stained with H&E for histopathological examination. Results: The mean values (I.U/L) of SGPT levels of albino rats in control groups A1, A2 and A3 were 40.83±1.62, 42.02±0.95 and 41.09±0.87 respectively, and in the tamoxifen-treated groups B1, B2 and B3 were 155.2±12.47, 243.60±11.96 and 277.60±12.84 respectively. This data show a highly significant increase (P<0.001) in the SGPT levels in all the B-subgroups (tamoxifen – treated) albino rats, in comparison with the control subgroups. The mean values (I.U/L) of SGPT in the tamoxifen plus taurine treated groups C1, C2 and C3 were 67.99± 1.89, 107.77± 2.28 and 137.93±8.29. Compared to the corresponding B subgroups, the data show highly significant decrease (P<0.001) in SGPT levels in groups C1 and C3, and moderately significant decrease (P<0.01) in SGPT levels in group C2. The mean values of serum levels (I.U/L) of SGOT in control groups A1, A2 and A3 were 38.66±0.89, 37.12±1.15 and 38.52±1.74 respectively. The mean values of SGOT in subgroups B1, B2 and B3 were 73.60±4.98, 152.48±13.00 and 247.40±18.53 I.U/L respectively. Thus there was moderately significant increase (P<0.01) in SGOT levels in B1, and highly significant increase (P<0.001) in B2 and B3 as compared to the control subgroups. The mean values of SGOT in subgroups C1, C2 and C3 were 65.20±3.15, 109.20±4.83 and 124.98 ± 5.83 respectively. The data show insignificant decrease in SGOT level in subgroup C1 (P>0.05), significant decrease in C2 (P<0.05), and moderately significant decrease (P<0.01) in C3 as compared to subgroups B1, B2 and B3, respectively. The mean values (I.U/L) of serum ALP in the control groups A1, A2 and A3 were 146.60±17.73, 196.40±22.47 and 164±24.60 respectively, and in the tamoxifen-treated groups B1, B2 and B3 were 436.80±30.92, 467.80±15.43 and 684.20±18.64 respectively. This shows highly significant increase in the serum levels of ALP (P<0.001) in all B subgroups. The mean values (I.U/L) of serum ALP in subgroups C1, C2 and C3 were 394.20±20.79, 376.60±20.02 and 420.00±14.66 respectively. This data show that there was insignificant decrease (P>0.05) in serum ALP in C1, moderately significant decrease (P<0.01) in C2 and highly significant decrease (P<0.001) in C3, when compared with B1, B2, and B3, respectively. Conclusion: Concomitant taurine administration lowers the mean values of serum hepatic enzymes in albino rats treated with heavy doses of tamoxifen.

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