Zohra R, Talat N, Aziz A.
A Comparative Study Of Operation Theatre Disinfection By Fumigation Using Different Compounds.
Biomedica Jan ;32(4):267-70.

Background and Objective: Objective of this study was i) to evaluate the efficacy of formalin and quaternary ammonium compounds (QUAT). ii) To compare the efficacy of both agents/techniques for operation theatre disinfection. This was an interventional, Case control study. This study was conducted at Children Hospital & Institute of Child Health Sciences, general operation theatre. Method: A total of 200 samples were collected out of which 80 samples (plates as well swabs) were collected before and after formalin fumigation and 120 samples prior and after spray fogging using QUAT based compound. The samples were cultured on blood and Mac-Conkey agar. Identification and isolation was performed in microbiology department according to bacteriological standards. Results: During fumigation by formaldehyde 34.3% samples (n = 32) were pre positive while 21.9% samples were post positive on different surfaces. On the other side 47.9% samples (n = 48) were pre positive while only 18.7% samples were post positive on different surfaces. Average bacterial count of air reduced from 744 (21 cfu/m3) to 329 (9 cfu/m3) after formalin vapor and 858 (25 cfu/m3) reduced to 492 (14 cfu/m3) after fogging. Conclusion: This study showed that fogging by less toxic compounds takes less time and has fewer disadvantages if we use automatic equipment having fine particle size.

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