Qureshi F, Tahir M, Lone KP.
Vitamins C and E Ameliorate the Sodium Arsenate Induced Interstitial Fibrosis and Tubular Atrophy in Fetal Kidneys of Albino MICE.
Biomedica Jan ;33(4):284-90.

Objectives: Epidemiological evidence indicates that exposure to arsenic contaminated drinking water during pregnancy may lead to various congenital abnormalities. The fetal renal tissue is impaired in relation to the developmental stage of kidney and to the amount and duration of arsenic exposure. This study evaluated the role of vitamin C and E in preventing the sodium arsenate induced mutilation of developing renal tissue of albino mice. Materials & Methods: Albino female mice of BALB/c strain (24), were randomly divided into 4 groups after confirmation of pregnancy. The control group A1 received 0.1 ml/kg body weight distilled water; group A2 was given a single dose of sodium arsenate (35 mg/kg) body weight on 8th gestational day (GD) by intra-peritoneal (I/P) injection. The groups A3 and A4 were treated with sodium arsenate (35 mg/kg) body weight on 8th GD and vitamin C, (9mg/kg/day) and vitamin E (15 mg/kg/day) by I/P injections, from 8th GD for the rest of the pregnancy period. The fetal kidneys were extracted on 18th GD. Three coronal sections stained with PAS and Masson?s trichrome from each kidney, were randomly chosen and examined for tubular injury and interstitial fibrosis. Results: The mean scoring for histological changes in fetal kidney tubules/10 fields among various study groups was statistically significant (p < 0.000). Group A2 showed severe tubular necrosis and interstitial fibrosis confirmed by Masson?s trichrome staining. Group A3 (vit C) and A4 (vit E) depicted mild to moderate improvement in tubular necrosis and interstial fibrosis. Conclusions: This study supports the role of vitamin C and E in ameliorating the developing renal tissue from the oxidative stress induced by sodium arsenate.

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