Sajjad A, Javed I, Mushtaq S, Anwer MS.
Frequency and Susceptibilty Patern of Non Fermenter Gram Negative Bacilli in a Tertiary Care Hospital.
Biomedica Jan ;33(3):174-8.

Background and Objective: There has been steady rise in imipenem resistant Gram negative bacilli non fermenters resulting from metallo beta lactamases that has been reported to be an important cause of hospital acquired infections and is serious therapeutic problem worldwide. The resistance produced by these pathogens has been mostly increased in Pseudomonas aeruginosa and Acinetobacter spp. during the last decade. These isolates pose not only a therapeutic problem but are also a serious concern for infection control management. This study was designed for the isolation and identification of non fermenter Gram negative bacilli in all the clinical samples and to determine their frequency and antibiotic susceptibility pattern. It was a Descriptive study and was performed in the Microbiology laboratory, Post Graduate Medical Institute (PGMI) Lahore from January 2015 to December 2015. Methods: All specimens obtained from Lahore General Hospital were processed in microbiology lab of Post Graduate Medical Institute, Lahore. Non fermenter Gram negative bacilli NFGNB were isolated by routine microbiological and biochemical procedures. Antibiotic susceptibility pattern was determined by Modified Kirby-bauer disc diffusion method. For this purpose all commercially available antimicrobial disks were used. Results: There were a total of 53 NFGNB that were isolated by convenient sampling. Respiratory samples were the most common specimens (39.6%) followed by urine (26.4%). Pseudomonas aeruginosa was the most common isolate (69.81%) followed by Acinetobacter baumannii (24.52%) and Pseudomonas luteola (3.77%). The maximum number of NFGNB were isolated in patients of age group above 60 years. Among NFGNB the maximum susceptibility was shown by Ciprofloxacin and Aztreonam whereas Acinetobacter junii showed 100% resistance. Conclusion: It is necessary to identify NFGNB in tertiary care hospitals to take strict infection control measures to limit the spread of the underlying resistance mechanisms caused by these pathogens and to monitor their susceptibility pattern to guide the clinician for better care and management of patients. They are now emerging as a cause of hospital and community acquired infections so their antibiotic sensitivity testing and infection control measures are needed to prevent the emergence and spread in health care settings.

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