Kafeel Ahmad, Amjad Ali, Shaista Rahat.
Prevalence of virulence genes among clinical isolates of Pseudomonas aeruginosa collected from Peshawar, Pakistan.
J Pak Med Assoc Jan ;68(12):1788-92.

Objective: To investigate six virulence genes in clinical isolates of pseudomonas aeruginosa collected from tertiary care hospitals. Methods: The cross-sectional study was conducted from December 2014 to June 2016 at the Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan and comprised pseudomonas aeruginosa isolates collected from Khyber Teaching Hospital, Peshawar, and Hayatabad Medical Complex, Peshawar, Pakistan. The isolates were recovered from pus, urine, sputum, wound, bronchial wash, cerebrospinal fluid, blood, high vaginal swab and diabetic foot. Genomic deoxyribonucleic acid was extracted from the isolates after identification and polymerase chain reaction technique was performed for the molecular detection of six virulence genes using specific primers. The six genes were: algD, lasB, toxA, plcH, plcN, and exoS Results: There were 182 pseudomonas aeruginosa isolates. The prevalence of algD was 179(98.3%), toxA 156(85.7%), lasB 179(98.3%), plcH 178(97.8%), plcN 170(93.4%) and exoS 175(96.15%). Conclusions: There was high prevalence of virulence factors among regional isolates of Pseudomonas aeruginosa.

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