Alveena Zehra, Sitwat Zehra, Muhammad Ismail, Abid Azhar.
Glutathione S-Transferase M1 and T1 Gene Deletions and Susceptibility to Acute Lymphoblastic Leukemia (ALL) in adults..
Pak J Med Sci Jan ;34(3):666-70.

Biotransformation of xenobiotics are critical for their metabolism and removal from the body which is carried out by xenobiotic metabolizing enzymes. Individuals carrying variants of genes that encode these enzymes have an altered ability to metabolize xenobiotics which may lead to an increased risk of acute lymphoblastic leukemia. The current study aimed to investigate the impact of and gene deletions in causing predisposition to adult ALL. The current case-control study involved 62 adult ALL patients and 62 age and gender matched healthy controls. Whole blood samples processed with standard phenol chloroform protocol for DNA isolation were genotyped using multiplex PCR approach for simultaneous identification of and deletions. The genotype frequency obtained for patients was compared to controls using odds ratio and chi-square. The null genotype frequency of and in a group of adult ALL patients from Pakistan were 47% and 11% respectively. Deletion of and did not show statistically significant association with adult ALL (=0.86 and =0.35 respectively). The combined deletion was observed in 2% patients and was not significantly associated with ALL in adults (=0.85). The results reveal that homozygous null polymorphism of and genes does not influence ALL susceptibility among adult patients. Cancer susceptibility associated with polymorphism varies with ethnic and geographic differences. Therefore, further investigation on different populations is needed to understand the role of these genetic variations in modifying adult ALL risk.

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