Muhammad Shahbaz Hussain, Azfar Sattar, Alia Batool.
Disruption of Pseudomonas aeruginosa and Staphylococcus aureusbiofilm by Manuka and Beri Honey.
Pak J Med Health Sci Jan ;12(1):142-6.

Aim : Formation and detection of the biofilm in microtiter plate and role of honey in prevention and disruption of this biofilm. Methods : A six months experimental study was carried out at the of Microbiology department university of Health Sciences (UHS) Lahore, Pakistan in which MDR clinical isolate of Staphylococcus aureus and Pseudomonas aeruginosa were used. Biofilm formation and detection was done by microtiter plate method. Two different honeys were used in order to see either they inhibits and disrupt the biofilm or not. Results : Manuka and Beri honey inhibited the biofilm formation of both organism 20% and 30% (w/v). Both honeys disrupted the established biofilm of at concentration greater than 40% (w/v). It was clear from results that a higher concentration of both honey was required to inhibit as well as disrupt established biofilm. Conclusions: Staphylococcus aureus and Pseudomonas aeruginosa form strongly adherent biofilm. The conclusion of this study is that honey whether local or from outside possesses good anti biofilm activity against MDR organism.

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