Sibgha Fatima, Zanib Arif, Humaira Gulnaz, Nabeela Kaukab, Sally Catt.
Toxic town: ART disinfectants damage human sperms: an investigation into potential toxicity of ART disinfectants on human sperms.
Pak J Med Health Sci Jan ;12(4):1413-5.

Aim: To investigate toxicity of disinfectants using human sperm motility assay (HSMA) in order to establish universal disinfection protocol, safe by all means for ART set up. Methods: Semen samples obtained from normospermic men and processed through density gradient, pellet was resuspended in 5ml of KSOM handling medium and motility assessed at 0 hours. The replicate splitted equally into five 1ml aliquots. In direct contact trial, four test tubes treated by adding 0.5ml of each disinfectant simultaneously (70% Ethanol, Fertisafe, Fertisafe Plus and Farmacidal) directly, one test tube without disinfectant being a control. Results: Human sperm motility was lost after 24hrs of direct contact trial treatment with disinfectants as compared to control (p<0.01). Sperm motility lost was more than 90 percent after 24hrs of direct contact trial of disinfectants. Conclusion: Significant association of disinfectant use in ART setup and loss of sperm motility was found in direct contact trial.

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