Farwa Naqvi, Nadeem Yaqoob, Maheen Rana, Shoaib Ahmed, Sultan Sikandar, Bilal Habib.
Anti-platelet effect of BryophyllumPinnatum Aqueous extract in human blood.
Pak J Med Health Sci Jan ;13(1):33-6.

Background: Bryophyllum pinnatum has demonstrated anti-inflammatory effect through arachidonic acid pathway. Blockage of arachidonic acid pathway causes reduced production of thromboxane A2, which may be responsible for its possible anti-platelet effect. Aim: To compare the effect of Bryophyllum pinnatum aqueous extracton platelet aggregation in vitro, by using arachidonic acid, thrombin and ADP as agonists. Study design: Experimental Study Setting: This study was conducted in Hematology Laboratory of Children Hospital, Lahore and Pharmacology Department of Post Graduate Medical Institute, Lahore. Method: Twenty healthy volunteers aged between 24-35 years from both genders, were selected from patient’s attendants and staff of Children Hospital. Their blood samples were evaluated for platelet count. Platelet rich plasma was prepared and platelet aggregation was checked with three different concentrations of Bryophyllum pinnatum by using ADP, arachidonic acid and thrombin. The data collected was processed by using Statistical Package for Social Sciences (SPSS 20), using one way ANOVA followed by post hoc Tukey’s test. A p value of ≤ 0.05 was considered significant. Results: This study demonstrated in vitro anti-platelet effect of Bryophyllum pinnatum , which was not dose dependent. Inhibition of platelet aggregation was marked with arachidonic acid, minimal with thrombin and negligible with ADP. Conclusion: The possible mechanism of anti-platelet effect of Bryophullum pinnatum is mainly through arachidonic acid pathway and partially through thrombin inhibition.

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