Zill-e Huma, Iffat Javed, Sohaila Mushtaq, Muhammad Saeed Anwar.
Frequency of Group D Streptococci Isolated from Uti Cases among Hospitalized patients and Their Antibiotic Sensitivity Pattern.
Esculapio J Services Inst Med Sci Jan ;13(4):222-6.

Objective: To determine the frequency and antibiotic susceptibility pattern of Enterococci obtained from UTI patients. Methods:Atotal of 2653 urine samples have been received for culture and sensitivity during the study period, 1387 were culture positive and 117 urine specimens have recovered Enterococci. The repeated urine cultures recovering Enterococci from the same patient and with mix growth were excluded from the study. The patients were asked to wash the perineal area with clean tap water. Females patients were instructed to apart the labia majora and minora to wash the area before collecting sample to avoid vaginal contamination.. Sterile plastic, wide mouthed containers were used to collect early morning, clean catch, mid-stream urine samples, and the samples were processed as soon as possible within 30 min. In case of delay, the samples were refrigerated. The urine samples were inoculated on Cysteine Lactose Electrolyte Deficient (CLED) media (Oxoid, England) and incubated at 37C for 24 hours. Bacterial growth was taken significant as per count (single species count > 105 organisms). The growth appeared was identified by colonial morphology, Grams Staining, Catalase Test, Bile Esculin test and growth in nutrient broth with 6.5 % NaCl. The antibiotic sensitivity pattern was determined by modified Kirby Bauer disc diffusion method. Results: The eighty six (73.50%) samples showed salt tolerance as growth appeared and turned out as Group D Enterococci whereas 31 (26.50%) did not show salt tolerance as no growth was observed and labelled as group D non Enterococci. The antibiotic sensitivity of the Group D Enterococci by Kirby bauer disk diffusion mehod with Penicillin 81(82.65%), Ampicillin 73(74.49%), Gentamicin 61(62.24%), Vancomycin 90 (91.84%), Linezolid 98(100%), Ciprofloxacin and Levofloxacin 55 (56.12%) and 57 (58.16%), Nitrofurantoin and Fosfomycin is 42 (42.86%) and 34 (34.69%). While antibiotic sensitivity of Group D non Enterococci with Penicillin 18(68.71%), Ampicillin 9(47.36%), Gentamicin 10(52.63%), Vancomycin 19 (100%), Linezolid 19 (100%), Ciprofloxacin and Levofloxacin is 9 (47.36%) and 9 (47.36%), Nitrofurantoin and Fosfomycin is 11 (57.89%) and 14 (73.68%). Conclusion: The frequency of the Group D Enterococci is 86 (73.50%) while that of Group D non Enterococci is 31 (26.50%). The Enterococci are most susceptible to Linezolid, Vancomycin, Penicillin, Ampicillin, Nitrofurantoin, Levofloxacin, Ciprofloxacin, Gentamicin and Fosfomycin in descending order while the non Enterococci are most susceptible to Linezolid, Vancomycin, Fosfomycin, Penicillin, Nitrofurantoin, Gentamicin, Ciprofloxacin and Levofloxacin in descending order.

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