Muhammad Waseem, Noreen Sarwar, Ayesha Sajjad, Rabiya Jamil, Muhammad Jawad Anwar.
Isolation of Gram positive and Gram negative bacteria from operation theaters of tertiary care hospital and molecular detection of staphylococcus aureus genes.
Pak J Med Health Sci Jan ;13(4):742-4.

Aim : To identify the microorganism s in different operation theaters in tertiary care hospital and characterization of isolates on the basis of biochemical test and molecular level. Methodology : Microbial contamination of five different operation theaters were selected including General surgery (OT1), gynecology (OT2), urology (OT3), orthopedics (OT4) and cardiac (OT5). Air samples of five different operation theatres were collected by Settle Plate Method. Sterile plates of nutrient, blood and Macc onkey’s agar were kept open for 15 min at four different places in operation theatres for air sampling. Result: Staph aureus was commonly found in OT’s and to confirm this bacteria, molecular identification was done by amplifying TS taG gene. Conclusion: Microbial burden is too much high in operation theatres and proper sterilization and fumigation is required.

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