Haroon Habib, Yasir Ali Bhatti, Kamran Arzoo, Muzamil Liaqat Ali, Ali Iftikhar, Mudaser Hussain Abbasi, Usama Ishtiaq, Mushtaq Fareed.
Sequels of methanolic Berberis vulgaris extract in cyclophosphamide induced hepatotoxicity in rats.
Pak J Med Health Sci Jan ;13(4):883-6.

Background: Cyclophosphamide (CP) is branded as an effective anti-cancer medication with hostile effects including induced hepatotoxicity, nephrotoxicity, cardiotoxicity and elevated cholesterol levels along with marked hematological effects. In this article, the protecting effects of Berberis vulgaris methanolic root extracts (Pre & Post treatment) on CP -induced ALT, AST variations in rats were calculated . Place of Study: Study was piloted in Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore. Duration of Study: 01 year from 01 January 2015 - 31 December 2015 Study Design: Observational type of descriptive study Methods /Results: Total 24 adult healthy male albino rats divided in six groups with four rats in each group (n=4) weighing between 120 -200g were housed in Animal House of The University of Lahore and permitted entree to saline and standard diet under measured settings of temperature 25±2 and photo period (12 hours dark and light) allowed, during the trial. Berberis vulgaris root extracts were prepared in 70% ethanol, filtered and concentrated to dry on rotary evaporator at 50°C and Cyclophosphamide was procured from Pharmedic Laboratories (pvt, limited), with dose of 1000mg/kg and 80mg/kg respectively, prepared in water for injection. The experimental rats were anesthetized by chloroform and then dissected. Blood was collected directly from Heart, then centrifuged right after and serum was separated for complete biochemical analysis, and aseptic measures were taken. For further use, -80°C was the temperate of tissue samples of liver, kidney, heart and brain storage in 10 % buffered formalin. Conclusion: It was determined that, B. vulgaris has probable ameliorative role in deterrence of onset and progression of cyclophosphamide -induced hepatotoxicity.

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