Anam Ali, Muhammad Asif, Iram Ali, Bismah Ahmad, Muhammad Tahir Khadim, Farhan Akhtar, Muhammad Sajjad Abbasi.
Analysis of CD10 expression in the epithelial lining of odontogenic cysts.
Pak Armed Forces Med J Jan ;70(Suppl-1):S10-14.

Objective: To investigate the expression of CD10 in the epithelium of Odontogenic keratocyst (OKC), Dentigerous and radicular cyst. Study Design: Cross sectional analytical study. Place and Duration of Study: Armed Forces Institute of Pathology, Rawalpindi, from Jan 2017 to Dec 2017. Methodology: In this study, total of sixty cases were included with 20 of each Radicular cyst, Dentigerous cyst and Odontogenic keratocyst. Sections were stained with Haematoxylin and eosin (H&E) followed by Immuno-histochemistry (IHC) staining for CD10 antibody. SPSS version 20 was used to analyse the results. Expression of CD10 was evaluated. Results: Out of total 60 cases, 41 (68.3%) were male and 19 (31.7%) were female patients with age ranges from 11 to 75 years. Mandible was reported in 38 (63.3%) of the cases followed by maxilla in 22 (36.7%) of the cases. In this study 7 (38%) cases of Odontogenic keratocysts showed negative epithelial CD10 expression as compared to Dentigerous cysts and Radicular cysts (45% & 25%). In Odontogenic keratocysts 9 (45%) of the cases showed basal layer of positivity of epithelium. While in dentigerous cyst 11 (55%) of the cases showed CD10 expression in the superficial layer of epithelium and the entire epithelium was positive in almost all 15 (75%) cases of radicular cyst. Intensity of staining was moderate in maximum number of the cases of Odontogenic keratocyst and radicular cyst (40% & 35%) as compared to dentigerous cyst (20%). There was a statistically significant association between odontogenic cysts and epithelial CD10 expression (p=0.001). Conclusion: Majority of odontogenic cysts showed positivity for CD10 marker. Odontogenic keratocysts showed basal, DCs showed surface and RCs showed full thickness positivity for this marker.

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com