Sher Zamir, Azhar Ijaz, Muhammad Umair, Rahat Jan Wazir, Mohammad Salman, Aamir Nazir.
Serum Concentration of Thyroid Hormones and Thyroid Stimulating Hormone in Alloxan-Induced Type 1 Diabetic Wistar Albino Rats.
Gomal J Med Sci Jan ;17(4):112-6.

Background: Literature has reported thyroid functional abnormalities in diabetes mellitus. The objectives of this study were to determine and compare the serum concentrations of T3 , T4 and TSH in alloxan-induced type 1 diabetic and control Wistar albino rats. Materials & Methods: It was an experimental animal study on 20 Wistar albino rats, extending over a period of eight weeks. Alloxan, a diabetogenic agent, was used to produce animal models of type 1 diabetes. Animals were divided equally into two groups: control and diabetic. The animals in the diabetic group were injected intraperitoneally with 150 mg/kg body weight of 10% alloxan to induce diabetes. After 72 hours, diabetes was confirmed with glucometer (glucose >350mg/dL). During the course of experiment, one rat in control group and 2 rats in diabetic group died. Blood was collected for estimation of serum concentrations of thyroid hormones, thyroid stimulating hormone at the end of experimental period. Serum T3 , T4 , and TSH were measured using ELISA kits. Results: At the end of eight weeks, the mean concentration of serum T3 was 0.69 +-0.29 ng/ml and 0.44+-0.02 ng/ml in control and diabetic groups, respectively. The mean concentration of T4 was 3.78+-1.16 ug/dL and 2.24+-0.86 ug/dL in control and diabetic groups respectively. The mean concentration of TSH was 0.77+-0.20 uU/ml and 1.41+-0.23 uU/ml in control and diabetic groups respectively. The mean serum concentrations of T3 (p=.0025) and T4 (p=<.00001) were significantly lower in diabetic and that of TSH (p=<.00001) were significantly higher in diabetic than control group. Conclusion: This study concludes that the serum concentrations of both T3 and T4 are significantly lower and that of TSH is significantly higher in alloxan-induced type 1 diabetic as compared to control group in Wistar albino rats.

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