Madiha Arshad, Shahida Niazi, Safeena Sarfraz.
Bone Tumors & Expression of SATB2 Immunohistochemical Stain in the Diagnosis of Osteogenic Sarcoma.
J Sharif Med Dent Coll Jan ;6(2):56-61.

Objective: To determine the frequency & histopathological features of various benign and malignant bone tumors at Mayo Hospital/KEMU, Lahore. To evaluate the expression of Special AT-rich sequence-binding protein 2 (SATB2), a new novel immunohistochemical stain in the diagnosis of osteogenic sarcoma. Methodology: It was a retrospective cross-sectional study of 83 cases of bone tumors received and reported from 1* July 2017 to 30** June 2019 at Mayo Hospital/ KEMU, Lahore. Relevant records & data, radiological findings, paraffin wax blocks & slides were retrieved and reviewed. Fresh slides were prepared wherever required. The immunohistochemical (IHC) stain of SATB2 was applied to the relevant malignant cases. Data was compiled & analyzed using Statistical Package for the Social Sciences (SPSS) version 23.0. Results: Out of a total of 83 bone tumors, 56(67.46%) cases were classified as benign & 27(32.53%) cases as malignant. Four cases in the malignant category were metastatic in origin. The commonest benign tumor was osteochondroma comprising of 24(42.85%) cases followed by giant cell tumor consisting of 16(28.57%) cases. The most frequent malignant bone tumor was osteosarcoma composed of 14(51.85%) cases & chondrosarcoma was the 2" commonest malignant tumor comprising of 5(18.85%) cases. In the benign cases, the age of the patients ranged from 12 to 47 years & in the malignant cases, the age ranged from 5 to 67 years. Nine (64.29%) cases of osteosarcoma were reported in children in the age range of 5 to 16 years. Overall humerus was the bone, most frequently affected by both benign & malignant bone tumors comprising 24(28.90%) cases followed by the femur which was affected in 21(25.3%) cases. Nuclear positivity for SATB2 was noted in 12(87.5%) cases of osteosarcoma. Conclusion: Osteochondroma is the commonest benign bone tumor and osteosarcoma is the most frequent malignant bone tumor. The immunohistochemical stain SATB2 proves to be a very effective tool in the diagnosis of osteosarcoma. Keywords: Bone tumors. Immunohistochemical stain SATB2. Osteosarcoma. Osteochondroma.

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