Butt T, Ahmad R N, Afzal R K, Mahmood A, Anwar M.
Rapid detection of rifampicin susceptibility of Mycobacterium Tuberculosis in sputum specimens by Mycobacteriophage assay.
J Pak Med Assoc Jan ;54(7):379-82.

Objective: To evaluate the performance of FASTPIaqueTB-RIF TM, a newly introduced bacteriophage assay for rapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens. Methods: A comparative study of 40 sputum specimens from patients of pulmonary tuberculosis, using FASTPIaqueTB-RIFTM and Bactec 460 TB system carried out at the Armed Forces Institute of Pathology, Rawalpindi between September and November 2001. Results: Of the 40 clinical isolates of Mycobacterium tuberculosis tested for rifampicin (RIF) susceptibility using the Bactec 460 TB system, 28 isolates were resistant to RIF and 12 isolates were susceptible. FASTPIaqueTBRIFTM identified 24 specimens as resistant to RIF. Three specimens that revealed susceptible isolates on Bactec 460, were resistant by FASTPIaqueTB-RIF TM while four specimens which revealed resistant isolates on Bactec 460, demonstrated susceptibility to RIF by FASTPIaqueTB-RIF TM. The sensitivity and specificity of FASTPIaqueTB-RIFTM were 86% and 73% respectively. The predictive values of positive and negative tests were 0.89 and 0.67 respectively. The overall accuracy of the technique was 82%. The phage assay took 48 hours to perform. Conclusion: Early detection of rifampicin resistance by the mycobacteriophage technique direct from sputum specimens is a potentially useful new test which would allow decision regarding appropriate therapy to be made early thus having a positive impact on patient care and on prevention of spread of MDR TB (JPMA 54:379;2004).

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com