Muhammad Azhar Khan.
Pituitary Vasopressin concentrations and water retention in ovariectomized rats treated with Oestradiol Benzoate.
Professional Med J Jan ;3(2):161-4.

Plasma vasopressin levels have been shown to vary throughout the rat oestrus cycle and following ovariectomy. To determine how the fluctuations in vasopressin are related to fluid balance, studies have been carried out in ovariectomized female Sprague-Dawley rats injected subcutaneously daily with either vehicle or oestradiol benzoate (50 g/rat) for 14 days. The animals were housed in individual metabolism cages under 12 h light/12 h dark regimen with free access to food and water. Blood samples to determine heamatocrit, blood volume, plasma volume and plasma osmolality were obtained. Pituitary fragments were taken to determine pituitary vasopressin concentration by radio-immunoassay. The results of this study indicate water retention in oestradiol benzoate-treated rats and oestradiol-induced increase in vasopressin may play a role in this fluid retention.

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