Saqib Siddiq, Mohammad Tayyab, Mahrukh Khan, Rashid Latif Khan.
Hypo-Osmotic swelling test.
Professional Med J Jan ;9(2):173-7.

Hypo-Osmotic Swelling (HOS) test is considered as a reliable method to find out live sperms. OBJECTIVE: To evaluate the effect of HOS test for selection of alive sperms. DESIGN: Prospective randomized study. PERIOD: June 2000-March 2002. SETTING: Infertility Advisory Centre Lahore. METHODS AND RESULTS: Twenty-four ICSI cycles were included in the study. In all the cases the sperms were non motile they were divided in two groups. In group A (12 cases) sperms were used which were tested by HOS and labeled as alive sperms. In group B (12 cases) in which sperms were randomly selected (without HOS Test) and used in ICSI Fertilization, embryo quality and clinical pregnancy rate were compared in two groups. CONCLUSIONS: HOS test is an effective and safe method to be used for selecting alive sperms in cases of necrospermia when they are used in Intracytoplasmic sperm injection (ICSI) to achieve a pregnancy.

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