Mohammad Ashraf Ganatra, Ghulam Qadir Fayyaz, Tahir Sheikh.
Vaginoplasty.
Professional Med J Jan ;12(4):404-7.

Objectives: To assess the efficacy of amniotic membrane as a true covering in vaginoplasty. Design: A prospective hospital based study. Setting: Department of Plastic Surgery Dow Medical College & Civil Hospital Karachi and Punjab Medical College & Allied Hospital Faisalabad. Period: From 1997 to 2002. Subjects & Material: All patients selected for vaginoplasty surgery were explained every detail about the procedure. Amniotic membrane was obtained from caesarean section placentas, washed with solution of normal saline, crystalline penicillin and sodium hypochlorite. A pocket was created between ,rectum and urinary bladder. Amniotic membrane was wrapped on vaseline bandage and inserted into neo vagina. First dressing was changed on 5th day and subsequent dressings with amnion were done on 7th 9th and 11th days. Results: Amnion was used in five patients. There was shrinkage of vaginal cavity in all these patients. Conclusion: Amniotic membrane should be avoided in reconstruction of vagina.

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